Coding
T5 Exonucl

Part:BBa_K3271025:Design

Designed by: George Liu and Setti Belhouari   Group: iGEM19_uOttawa   (2019-10-16)


T5 Exonuclease in pSB1K3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Coding sequence was codon-optimized for expression in E. coli and for the removal of illegal restriction sites.


Source

The coding sequence was derived from the genome of T5 phage.

Reference for original sequence (prior to our adjustments)

Kaliman, A. V., Krutilina, A. I., Kryukov, V. M., & Bayev, A. A. (1986). Cloning and DNA sequence of the 5'-exonuclease gene of bacteriophage T5. FEBS Letters, 195(1-2), 61-64. doi:10.1016/0014-5793(86)80130-2 T

References