![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
T5 Exonucl
Part:BBa_K3271025:Design
Designed by: George Liu and Setti Belhouari Group: iGEM19_uOttawa (2019-10-16)
T5 Exonuclease in pSB1K3
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Coding sequence was codon-optimized for expression in E. coli and for the removal of illegal restriction sites.
Source
The coding sequence was derived from the genome of T5 phage.
Reference for original sequence (prior to our adjustments)
Kaliman, A. V., Krutilina, A. I., Kryukov, V. M., & Bayev, A. A. (1986). Cloning and DNA sequence of the 5'-exonuclease gene of bacteriophage T5. FEBS Letters, 195(1-2), 61-64. doi:10.1016/0014-5793(86)80130-2 T